01. ZZ Top - La Grange (3:51)
02. Foghat - I Just Want to Make Love to You (4:21)
03. Buddy Guy, Junior Wells - Messin' with the Kid (2:16)
04. Robben Ford - Talk to Your Daughter (4:11)
05. Grateful Dead - Truckin' (5:07)
06. The Band, Muddy Waters - Mannish Boy (Live) [feat. Muddy Waters] (6:41)
07. David Lindley - Mercury Blues (3:35)
08. The J. Geils Band - Whammer Jammer (2:38)
09. John Hammond - Evil Is Going On (3:40)
10. Freddie King - Stumble (3:37)
11. Aretha Franklin - Why I Sing the Blues (3:05)
12. Kenny Wayne Shepherd - Be Mine (4:08)
13. Johnny Winter - Third Degree (6:35)
14. Dr. John - Wash, Mama Wash (3:40)
15. The Robert Cray Band - All the Way (5:12)
16. The Paul Butterfield Blues Band - Born In Chicago (3:14)
17. Buddy Guy - Mary Had a Little Lamb (2:34)
18. Canned Heat - Bagful of Boogie (3:35)
19. Tony Joe White - Boom Boom (7:52)
20. Champion Jack Dupree - Junker's Blues (3:13)
21. James Cotton, Joe Bonamassa - Cotton Mouth Man (feat. Joe Bonamassa) (3:47)
22. Taj Mahal - Queen Bee (4:28)
23. Beth Hart - Am I the One (6:39)
24. Albert Collins - Ice Pick (3:09)
25. Brownsville Station - Smokin' In the Boy's Room (2:58)
26. Professor Longhair - Big Chief (3:16)
27. Mississippi John Hurt - I'm Satisfied (2:54)
28. Faces - You're So Rude (3:44)
29. Charlie Musselwhite - I Don't Play, I'll Be Your Man Some Day (3:19)
30. Jesse Winchester - Step By Step (2:57)
31. Kelly Joe Phelps - Goodnight Irene (5:40)
32. Otis Redding - Satisfaction (I Can't Get No) (2:45)
33. T-bone Walker - How Long Blues (5:17)
34. Nina Simone - Gin House Blues (3:04)
35. The Nashville Teens - Hoochie Coochie Man (3:38)
36. Etta James - Lovesick Blues (3:36)
37. Small Faces - You Ain't Seen Nothing Yet (2:59)
38. The Yardbirds - Drinking Muddy Water (2:57)
39. Ray Charles - Blackjack (2:21)
40. Mable John - Your Good Thing (Is About to End) (2:59)
41. Doug Sahm - Dealer's Blues (2:59)
42. Lou Johnson - Rock Me Baby (2:15)
43. Little Richard - Brown Sugar (3:27)
44. Bonnie Raitt - Finest Lovin' Man (4:42)
45. James Gang - Standing In the Rain (5:07)
46. Blues Image - Lazy Day Blues (4:50)
47. Allen Toussaint - On Your Way Down (3:58)
48. John Baldry - I'm Ready (4:13)
49. Johnnie Taylor - I Got to Love Somebody's Baby (3:01)
50. The Boogie Kings - Sweet Home Chicago (Live) (2:48)